FastQCFastQC Report
Sun 14 Apr 2019
SRR6576913_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576913_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54120
Sequences flagged as poor quality0
Sequence length100
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1350.24944567627494454No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT640.11825572801182557No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT560.10347376201034737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA2000.063.4918371
ATCAACG2550.049.7514953
TATCAAC2650.047.9183652
TCAACGC2650.047.8740844
GGTATCA708.362622E-847.030991
GTATAAT405.6103064E-447.0309871
CAACGCA2700.045.2472465
GTACATG4900.042.231911
CATGGGA2450.042.192884
AACGCAG2900.042.1267436
TACATGG4500.041.8053252
ACATGGG4800.040.1351743
TAATATA608.726691E-539.156274
ACGCAGA3400.035.9316377
CGCAGAG3400.035.9316378
ATGGGAG1054.97148E-835.800025
ATGGGGG801.1404767E-535.2406435
GCAGAGT3700.031.7483259
ATGGGAT1052.0218995E-631.3250145
CATGGGG2000.030.5418914
Produced by FastQC (version 0.11.2)