FastQCFastQC Report
Sun 14 Apr 2019
SRR6576920_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576920_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1053868
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13020.1235448841790433No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA12690.12041356222980487No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA12260.11633335484140328No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC11910.11301225580433225No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT11890.11282247871649961No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11520.10931160259159592No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT10700.10153074199045801No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10660.10115118781479275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25350.058.969331
GGTATCA9450.047.7547381
ATCAACG33500.044.1936953
CAACGCA35000.042.299685
TCAACGC35050.042.239344
AACGCAG37100.040.0320436
TATCAAC38850.038.3570562
ACGCAGA43000.034.320677
CGCAGAG43950.033.5788158
GCAGAGT48300.030.4573259
GTACATG51500.028.935371
TAGTACG650.006154166528.922864
TACATGG51550.028.7249242
GTATAAT3400.027.6520981
ACATGGG53300.027.159273
TAGACCG700.0088360226.856945
GTACTAG3000.026.6381871
AGAGTAC44750.025.46908210-11
CATGGGG26800.024.2013114
AGTACTT19350.021.61740712-13
Produced by FastQC (version 0.11.2)