FastQCFastQC Report
Sun 14 Apr 2019
SRR6576920_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576920_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1053868
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14330.13597528343208068No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA11740.11139915055775484No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT11200.10627516918627382No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11080.10513650665927801No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC10820.10266940451745381No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10600.10058185655129484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25300.059.2121961
GGTATCA9900.053.2951131
ATCAACG33950.043.3309563
CAACGCA35200.041.525175
TCAACGC35150.041.4505274
AACGCAG37650.038.9478386
TATCAAC38850.038.7126272
ACGCAGA43950.033.4702157
CGCAGAG44950.032.8301668
GTACATG51500.030.277871
TACATGG51550.030.0870062
GCAGAGT48850.030.0167129
ACATGGG52400.028.7917083
CATGGGG29050.026.5333084
AGAGTAC46300.024.76748710-11
GGGTACC6150.022.1613377
AGTACTT20450.021.94740312-13
CTAGTAC3650.021.89023
TATCGGC1354.077005E-420.8886892
CAGAGTA47600.020.63537610-11
Produced by FastQC (version 0.11.2)