FastQCFastQC Report
Sun 14 Apr 2019
SRR6576921_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576921_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1038865
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14610.14063424987847314No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC11640.1120453571927055No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA11060.1064623411126566No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10600.10203443180779025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24850.057.013031
GGTATCA10400.048.879241
ATCAACG31800.043.738753
CAACGCA32700.042.391235
TCAACGC33250.041.831354
AACGCAG34750.039.8942956
TATCAAC37550.037.541632
ACGCAGA40350.034.2410747
CGCAGAG40650.034.011298
GCAGAGT45350.030.5901139
TACATGG49350.029.7077162
GTACATG51600.028.7339171
ACATGGG51850.027.1878133
AGAGTAC43000.025.31738910-11
CATGGGG28350.024.8622254
TACACCG2453.4015102E-1023.0153165
ATATACG4900.022.0584686
ACCGTCC1100.003049617221.3753728
ACGCCTA1554.2420634E-521.2231526
AGTACAT34100.021.03060712-13
Produced by FastQC (version 0.11.2)