FastQCFastQC Report
Sun 14 Apr 2019
SRR6576922_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576922_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1139237
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA57930.5084982317112243No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC41500.36427889894727783No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC37060.32530544566231606No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC20140.17678498854935365No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG19690.172834976392094No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC17180.15080268635937913No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT16450.1443948888598246No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT16260.14272710594898164No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG15470.13579264016179252No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA14910.1308770694772027No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA14420.12657594512818668No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC14230.12490816221734372No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13830.12139704029977959No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT13370.11735925009458084No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA13140.11534035499198146No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT12940.1135847940331994No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC12550.11016145016357438No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA12120.10638699410219295No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA12120.10638699410219295No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT11980.10515810143104552No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT11800.10357809656814165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6500.062.1887661
GGTAATC5300.048.772568
GTATCAA25550.045.0715031
ATCAACG26600.042.051753
GTAATCA6200.041.6926739
TCAACGC27000.041.4287644
CAACGCA27450.040.9208155
AAGGGTA6350.040.7078065
AACGCAG28700.039.302316
GTGCAAG6950.036.5204241
TATCAAC32150.035.3802762
AGGGTAA7450.035.3281146
GTCTTAG14350.034.064991
CTTAGGT13500.032.7252433
TTAGGTA13550.032.604484
TAGGTAT13800.032.3543935
GTACATG46400.032.1120571
TGCAAGG8200.032.0997052
AGGTATA14200.031.4436
TACATGG47450.031.3024062
Produced by FastQC (version 0.11.2)