FastQCFastQC Report
Sun 14 Apr 2019
SRR6576922_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576922_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1139237
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA62080.544926121605952No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC42270.3710378086385888No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC39770.34909329665381306No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG18750.16458383988581832No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC18680.1639693935502446No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC17400.1527338034140394No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT16690.14650156201036307No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG16420.1441315547160073No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT16200.14220043766134702No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG15410.13526597187415787No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15220.13359818896331493No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA14860.13043817923750722No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT14770.12964817680605528No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA13510.11858814276572828No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC13140.11534035499198146No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT12980.11393590622495584No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA12880.1130581257455648No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA12640.11095145259502631No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT12040.10568476971868014No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA11930.10471921119135No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT11740.10305142828050703No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC11470.10068142098615125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6100.063.3293381
GTATCAA26350.051.4911731
ATCAACG28800.046.1872863
TCAACGC28850.045.7813954
CAACGCA29950.044.2568785
AACGCAG30800.043.188116
TATCAAC32500.040.929042
ACGCAGA39250.033.8873147
TAGGTAT12050.032.7657975
GTACATG51700.032.439971
TTAGGTA12350.032.350464
CGCAGAG41300.032.3190548
AGGTATA12250.031.8471436
TACATGG52850.031.7505842
ACCTAAG21800.031.1190931
GTCTTAG13350.031.0543421
CTTAGGT12700.030.71873
ACATGGG53400.030.5433523
GCAGAGT44600.030.4532939
CTAAGAC22400.030.0065823
Produced by FastQC (version 0.11.2)