FastQCFastQC Report
Sun 14 Apr 2019
SRR6576924_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576924_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48308
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1260.2608263641632856No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT660.13662333360934006No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.10971267698931854No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG500.10350252546162127No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG5400.061.9217721
ACATGGG5650.059.8911673
TACATGG5600.059.7102852
CATGGGA3200.058.747414
CTATTGG250.0051641256.514521
CATGGGG2000.056.3975144
ACGCTGA250.005206718656.3975146
GTATCAA1600.055.925831
ATGGGAG1500.053.264325
ATGGGGC558.637726E-751.270475
ATGGGGG957.2759576E-1249.4715045
TGGGAGA1050.049.2359286
ATGGGAC555.2093645E-542.725395
ATGGGAT952.046545E-839.5772065
ATGGGGA608.7013905E-539.1649445
ATCAACG2350.037.9983253
AACGCAG2350.037.9983256
GGGAGAA500.001686302137.5983437
TGGGGGG757.2990133E-637.5983436
ATGGGAA1401.2732926E-1136.9269455