FastQCFastQC Report
Sun 14 Apr 2019
SRR6576924_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576924_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48308
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2300.4761116171234578No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT980.20286494990477766No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT760.1573238387016643No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATTC250.0052080856.393793
GTATCAA1650.054.283841
CTAATAC352.910948E-453.7083794
CCTACTT205.653856E-446.99482764-65
GGATAAC252.9907922E-546.99482370-71
CATGGGG1900.044.5214164
ATGGGGA652.731731E-643.3798335
ATGGGAC555.2113974E-542.722575
GTACATG4750.042.6752171
ATGGGAT1006.7666406E-1042.295345
ACATGGG4850.041.6655123
TACATGG4950.040.8237842
GGTATCA757.147659E-637.712981
ATGGGGT757.3023275E-637.595865
CTAGAGC250.001695239137.5958688-89
TACTTGG250.001695239137.5958666-67
CGCTGCT250.001695239137.5958690-91
CATGGGA2550.036.8586854
ATCAACG2650.033.69443
TATCAAC2700.033.070432
Produced by FastQC (version 0.11.2)