FastQCFastQC Report
Sun 14 Apr 2019
SRR6576925_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576925_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1167225
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA33860.290089742765962No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC25250.21632504444301656No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC19040.16312193450277368No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14880.12748184797275589No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA13490.11557326136777399No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG13290.11385979566921545No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC12570.10769131915440468No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12570.10769131915440468No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC12330.10563516031613443No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT12140.10400736790250381No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11970.10255092205872904No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT11910.10203688234916147No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11780.10092312964509842No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6400.056.555481
GTATCAA25350.054.5171741
ATCAACG27250.050.362343
TCAACGC27600.049.7236864
CAACGCA28800.047.6518675
AACGCAG29900.046.0559736
TATCAAC34750.040.0406272
ACGCAGA38150.036.0962947
CGCAGAG38900.035.400358
GCAGAGT41100.033.7341469
TAGGTAT8100.031.3327335
TTAGGTA8250.030.7630484
GTCTTAG9550.030.0255151
TACATGG45500.029.7539792
GTACATG46200.029.710151
AGGTATA8550.029.6836436
CTTAGGT8600.028.9645613
CTAAGAC13350.027.8122023
ACATGGG49150.027.4440353
TCTTAGG11050.026.800462
Produced by FastQC (version 0.11.2)