FastQCFastQC Report
Sun 14 Apr 2019
SRR6576925_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576925_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1167225
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA36530.31296450984171864No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC24150.20690098310094454No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC18390.15755317098245838No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13650.11694403392662084No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA12780.10949045813789114No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC12140.10400736790250381No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC11890.10186553577930563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6200.061.5323331
GTATCAA20250.051.866851
TCAACGC25700.039.867674
ATCAACG26200.039.2862243
CAACGCA27300.037.8754275
AACGCAG28700.036.3553666
CTTAGGT8600.035.5232243
GTCTTAG9650.034.653021
TTAGGTA8750.034.377114
TAGGTAT8750.034.377115
AGGTATA9150.032.8742876
TATCAAC32500.032.1045842
GTACATG45500.031.364711
GGTATAG9600.031.3319637
TACATGG46300.030.5550462
TAAGACA16650.029.9218944
TTAAGAC3150.029.8412423
ACGCAGA35150.029.682917
CTAAGAC14550.029.395163
ACATGGG47800.029.3012313
Produced by FastQC (version 0.11.2)