FastQCFastQC Report
Sun 14 Apr 2019
SRR6576926_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576926_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1099412
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA32990.3000694916919226No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC24630.22402884450961058No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC19540.17773136913186321No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15230.13852859528547987No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA12570.11433384390928969No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC12360.11242373195853783No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG12170.1106955354316671No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT11680.10623860754657945No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC11620.10569286127493606No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11540.10496519957941153No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC11380.1035098761883625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6400.064.6425861
GTATCAA20950.050.042251
TCAACGC24050.042.5893064
ATCAACG24600.041.6371083
CAACGCA24850.041.2182245
AACGCAG25800.039.7004936
TATCAAC29800.034.7074242
GTACATG40350.032.6234971
TACATGG40750.032.072532
ACGCAGA31950.032.0586177
ACCTAAG12550.031.841331
CTAAGAC13600.031.4384883
ACATGGG42350.030.5097353
TAAGACA15450.030.4109974
CGCAGAG34400.029.7753728
CCTAAGA15300.027.347312
GCAGAGT37800.027.0971649
ACCAGAT16450.025.99169594
CATGGGG25750.025.910174
GGTAATC3650.024.457948