FastQCFastQC Report
Sun 14 Apr 2019
SRR6576926_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576926_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1099412
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA35430.3222631734054203No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC23560.2142963693319702No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC17410.15835737648852297No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12440.11315139365406235No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT11520.1047832841555304No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC11510.10469232644358985No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA11480.10441945330776817No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC11290.10269125678089741No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6650.062.272331
GTATCAA20250.052.5191271
TCAACGC24050.043.7697534
ATCAACG24150.043.5885053
CAACGCA24900.042.2755975
AACGCAG25800.040.983026
TATCAAC30350.035.3050652
GTATATG1606.002665E-1132.352421
ACGCAGA33050.031.9913397
TAGGTAT7250.030.4649855
CGCAGAG34750.030.4498378
CTTAGGT7250.029.8167933
TTAGGTA7750.029.7122484
TACATGG41950.029.687582
GTCTTAG9250.029.506681
TGCGTTA404.7872218E-429.36982590-91
GTACATG42800.029.2463681
ACTACCG650.006136120328.9403694
GCAGAGT37000.028.8523649
AGGTATA7800.027.7143276