FastQCFastQC Report
Sun 14 Apr 2019
SRR6576935_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576935_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences267780
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7450.27821345880947046No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3340.12472925535887669No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG2980.1112853835237882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9550.045.8895341
ACCGTGA500.001701273437.5929038
GGTATCA4350.036.831871
ATCAACG12550.034.447683
CAACGCA12650.034.175375
TCAACGC12800.033.774874
AACGCAG13050.033.487936
TATCAAC13300.032.157832
TACATGG19100.031.7433912
GTACATG20000.030.6300321
ACATGGG19950.029.9141543
ACGCAGA15500.027.891517
CGCAGAG15700.027.5362038
TATAATG1205.821352E-627.4166132
AAATTCG350.00883196926.8570950-51
ATGGGAG4850.026.160015
CATGGGA13000.025.6643874
CAGTACT1501.1588727E-625.0619374
TAGGCCC950.001303407724.7321724
GTCCTAC1151.3491731E-424.585981
Produced by FastQC (version 0.11.2)