FastQCFastQC Report
Sun 14 Apr 2019
SRR6576936_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576936_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222077
Sequences flagged as poor quality0
Sequence length100
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5860.26387244063995824No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4930.2219950737807157No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3680.1657082903677553No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3330.14994799101212644No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3020.13598886872571225No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2990.13463798592380122No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2770.12473151204312018No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA2730.12293033497390544No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2710.12202974643929808No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2580.1161759209643502No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2530.11392444962783178No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT2370.10671974135097285No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT2320.10446827001445444No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2260.10176650441063234No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2230.10041562160872129No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2230.10041562160872129No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC2230.10041562160872129No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAA200.002163860270.473824
CGCTAAA200.002163860270.473825
CTTACTC352.937725E-453.694343
GTATCAA7050.046.0140041
ATGGGTA2250.039.6741525
CTACACT608.820371E-539.1521264
GGTACCT2200.038.4402668
GTACATG15650.037.8517761
GGTATCA3250.037.6114461
ACATGGG16100.037.060773
TGGGTAC2300.036.768956
CTTACTA1152.764864E-936.768959
CAACGCA9200.035.236915
ATCAACG9200.035.236913
TACATGG17100.035.1919982
AACGCAG9250.035.046446
TCAACGC9250.035.046444
CATGGGT3450.034.0453264
ATGGGAT2800.033.5589645
ACAGTCC702.184111E-433.5589648
Produced by FastQC (version 0.11.2)