FastQCFastQC Report
Sun 14 Apr 2019
SRR6576936_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576936_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222077
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6640.2989953934896455No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5580.2512642011554551No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3460.1558018164870743No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3350.1508485795467338No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3210.14454445980448224No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3030.13643916299301595No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2710.12202974643929808No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT2670.12022856937008335No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2620.11797709803356493No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2580.1161759209643502No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT2460.110772389756706No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2450.11032209548940232No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2280.10266709294523971No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACGA250.005233569556.391626
TCTATAC405.670923E-446.993023
GTATCAA10850.046.9668241
TCAACGC13850.036.9836774
CAACGCA13800.036.7771455
ATCAACG13950.036.381693
AACGCAG14250.035.615766
GGTATCA4800.034.4051931
GGTACCT2350.033.994958
GGGTACC2350.033.994957
CATGGGT4200.032.447564
TATCAAC15950.032.128822
ACGCAGA15850.031.7239957
CGCAGAG15900.031.6242338
GTACTGG1351.2676537E-831.4561791
ATGGGTA2700.031.3286825
ATGGGAT1807.2759576E-1231.328685
TGGGTAC2650.030.1464656
GCAGAGT17250.029.1492929
GTACCTG2750.029.0502289
Produced by FastQC (version 0.11.2)