FastQCFastQC Report
Sun 14 Apr 2019
SRR6576945_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576945_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences782661
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA30030.38369102331660837No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC18840.2407172453974326No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC18220.2327955526083451No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC12140.1551118555798743No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11660.14897893213025817No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG8710.1112870067628258No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC8690.11103146828575845No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT8480.10834831427655141No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA7950.10157654463426695No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4500.064.771761
GTATACG250.005236853456.396093
GTATCAA17750.054.295271
TCAACGC22550.041.6822554
ATCAACG22950.040.7509883
CAACGCA23500.040.3971985
AACGCAG23650.039.942266
CTATACA1550.039.4166224
TATCAAC27250.034.8490832
GGGTACC5250.034.016697
GTACATG36500.034.0030251
GGTACCT5250.033.1215138
TACATGG37650.032.464962
ACGCAGA29450.032.0758747
TGGGTAC6050.031.8490336
CGCAGAG30000.031.4878168
ACATGGG39100.031.0106373
CTTAGGT6700.030.8635333
ACCTAAG12150.029.0196041
GTACCGT650.006155069528.921076
Produced by FastQC (version 0.11.2)