FastQCFastQC Report
Sun 14 Apr 2019
SRR6576946_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576946_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences756547
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA29250.3866250213139435No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC19230.25418116785870537No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC18760.24796873161878905No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC12440.16443129111608398No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10070.1331047509275696No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT10070.1331047509275696No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC9240.12213385288686623No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG7890.10428962113391502No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7590.10032423629992583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4450.068.6553041
GTATCAA16050.050.9559561
TCAACGC17850.044.486244
CAACGCA18450.043.0395365
ATCAACG18500.042.923213
AACGCAG19500.040.722026
TATCAAC22300.035.8314862
GTACATG30400.033.7057271
TACATGG31250.032.4881132
TAGGTAT6700.032.2596975
ACGCAGA24850.031.9549087
ACCTAAG11000.031.1925531
CGCAGAG25800.030.778278
ACATGGG32600.030.700063
AAGGGTA3650.029.6082135
CTTAGGT7300.029.6082133
CTAAGAC12050.029.2449873
CCTAAGA12750.028.7544572
ATTATCC12800.028.632673
GTCTTAG7950.028.3788551