FastQCFastQC Report
Sun 14 Apr 2019
SRR6576946_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576946_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences756547
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA29450.3892686112032696No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC19680.26012924510968916No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC18030.23831962852274877No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC11200.1480410338022621No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT10290.13601269980582834No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9430.12464526328172605No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC9000.11896154501967492No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8480.11208821130742704No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG7640.10098513377225739No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4700.072.1200561
GTATCAA17250.048.8523371
ACGGTAA450.001010053341.80868594
ATCAACG19600.041.713963
TCAACGC19650.041.6078154
CAACGCA20300.040.275555
AACGCAG21150.038.879076
TATCAAC22100.037.4253652
GTACATG29350.032.561871
ACGCAGA25500.032.246767
TACATGG29600.032.2292332
CGCAGAG26100.031.5262938
ACATGGG30450.031.01683
GCAGAGT28250.029.6262659
GGATAAT5000.029.1885911
CTATTAA1853.2196112E-1027.9925441
TAATAGT5600.027.6894364
CATGGGG18100.027.5179144
CTTAGGT7600.027.2036573
GTATTAC700.00876265126.9019281