FastQCFastQC Report
Sun 14 Apr 2019
SRR6576947_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576947_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202552
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6910.34114696473004463No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4730.23352028121173823No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3340.1648959279592401No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3200.15798412259567912No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3160.15600932106323315No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2500.12342509577787432No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2400.11848809194675936No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2240.1105888858169754No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2190.10812038390141791No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2160.10663928275208343No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT2150.10614558236897192No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA2120.10466448121963742No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2080.10268967968719143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8050.054.957481
ATCAACG10150.042.133393
CAACGCA10650.041.4791075
TCAACGC10550.040.981374
AACGCAG11100.039.374146
TATCAAC11300.038.6772542
GTATAAA1204.114554E-935.298491
GGTATCA3000.034.5140761
TAGTACT550.002713184334.1781354
ACGCAGA12850.034.01197
CGCAGAG13150.033.2359668
GCAGAGT13950.031.3299629
GCCGTTC600.004155156731.3299628
GTACATG13250.030.1924171
ACATGGG13500.029.9375173
GTATTAG953.702285E-529.7250391
CATGGGG4700.028.9968784
TACATGG13800.028.9461572
GGTACCT2002.5465852E-1128.1969648
GCTCTTA856.7421544E-427.6850851
Produced by FastQC (version 0.11.2)