FastQCFastQC Report
Sun 14 Apr 2019
SRR6576953_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576953_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences915995
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA47780.5216185677869422No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC21230.2317698240710921No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG17680.1930141540073909No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC17360.18952068515657836No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC17150.18722809622323267No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA13950.15229340771510763No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA12760.13930207042614862No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12430.13569943067374823No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG11410.12456399871178336No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC10920.11921462453397672No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10360.11310105404505483No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC10210.11146349052123647No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT10080.1100442688005939No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC9970.1088433888831271No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT9840.1074241671624845No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9380.10240230568944154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6100.068.562141
GTATCAA26550.056.638221
GCGTAGT405.68086E-446.9920271
TCAACGC33050.044.356774
ATCAACG33750.043.436783
CAACGCA34650.042.4441535
AACGCAG36150.040.812966
AAGGGTA4200.040.2744875
TATCAAC36250.040.1862872
GGTAATC4450.038.011878
GTGCAAG5200.036.1477131
ACGCAGA43300.033.8566137
CGCAGAG44050.033.2801638
GCAGAGT45850.031.9736379
GTACATG51900.031.328021
ATTATCC10350.031.32463
TACATGG53500.030.12762
AGGGTAA6000.029.758376
TATTATC10900.029.7472462
ACATGGG53300.029.6202583
Produced by FastQC (version 0.11.2)