FastQCFastQC Report
Sun 14 Apr 2019
SRR6576953_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576953_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences915995
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA51490.5621209722760496No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC21610.23591831833143195No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG17070.18635472901052955No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC16990.1854813617978264No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG16570.180896183931135No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC15900.17358173352474632No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14320.15633273107385956No Hit
GACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGC12170.13286098723246306No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA11930.13024088559435368No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA11840.12925834748006268No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC10830.1182320864196857No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC10510.1147386175688732No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10430.11386525035617005No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC10270.1121185159307638No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA9430.10294816019738098No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA9290.10141976757515052No Hit
ACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA9280.10131059667356263No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA9180.10021888765768372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6700.072.372481
GTATCAA28100.057.4643941
ATCAACG33450.047.203173
TCAACGC33500.047.1327174
CAACGCA34500.045.4941335
AACGCAG36100.043.4777686
TATCAAC37650.042.0668832
ACCTAAG16100.037.1354681
ACGCAGA42350.037.0613337
CGCAGAG43250.036.2051778
CTAAGAC17200.035.244333
GCAGAGT44800.035.0575079
CCTAAGA18150.033.4032332
GTACATG50050.032.1686671
TAAGACA20650.032.086854
TACATGG51900.030.9694982
ACATGGG52550.030.4042433
ATGGGGG8450.030.030675
AAGGGTA3600.030.0229475
GTAATCA4800.029.3895249