FastQCFastQC Report
Sun 14 Apr 2019
SRR6576954_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576954_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1005232
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA36820.366283604182915No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC19750.19647205819154187No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC15910.15827192130771803No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13180.13111401149187452No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC11860.11798271443806006No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT10830.10773632355515941No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG10510.10455297881484076No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG10370.10316026549095135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6850.059.0097351
GTATCAA21800.054.9793361
TCAACGC26250.044.9383354
ATCAACG27350.043.1309473
CAACGCA27650.043.0029225
AACGCAG28150.042.23916
TATCAAC30500.039.2967032
ACGCAGA35300.033.683597
CGCAGAG36650.032.5710958
GTACATG47050.032.3669051
TAGGTAT6700.032.266785
ACCTAAG11300.032.0278741
TACATGG46600.031.3682462
CTAAGAC12200.031.2031023
GCAGAGT39900.029.9180589
TAAGACA14650.029.513644
ACATGGG49600.029.468043
CTTAGGT7400.028.5794163
TTAGGTA7900.028.5553024
CCTAAGA13600.028.3394072