FastQCFastQC Report
Sun 14 Apr 2019
SRR6576955_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576955_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1007941
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA37410.3711526765951578No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC20590.20427782975392408No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC16620.1648906037158921No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC11850.11756640517649347No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT11770.1167727079263568No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11720.1162766471450214No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG10850.10764518954978516No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG10110.10030348998602101No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5250.068.102151
GTATCAA20000.055.7474371
TCAACGC25100.043.061234
ATCAACG25600.042.220193
CAACGCA26400.041.29685
AACGCAG27150.040.3290866
TATCAAC31000.035.1723752
TAGGTAT7200.032.633965
ACGCAGA33550.032.355777
GTACATG41850.031.9248921
CTTAGGT7550.031.7435533
GTCTTAG8300.031.7406981
CGCAGAG34750.031.258618
TACATGG42600.031.1110672
TTAGGTA7800.030.7261314
ACATGGG43550.029.7819583
ACCTAAG13050.029.199881
TCTTAGG10200.028.567152
TAAGACA15300.028.2571744
GCAGAGT38450.028.2506289