FastQCFastQC Report
Sun 14 Apr 2019
SRR6576956_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576956_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700878
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA44570.6359166645264939No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC24300.3467079862686516No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC23840.3401447898207677No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC18520.2642399961191534No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG13680.19518375523272238No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC12150.1733539931343258No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10920.15580457654541877No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA10280.14667317279184108No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT10260.1463878164245418No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC10090.14396228730249772No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT9660.13782712540556274No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA9580.13668569993636553No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC8960.12783965255008717No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT7840.11185969598132628No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT7590.108292741390085No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG7580.10815006320643536No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT7520.10729399410453747No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA7500.10700863773723815No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT7400.10558185590074164No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA7230.10315632677869759No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG7070.10087347584030316No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA7030.10030276310570457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5250.072.532341
GTATCAA19150.048.1164171
TCAACGC22750.039.664494
ATCAACG22900.039.404683
AACGCAG23600.039.0324756
CAACGCA23700.038.6694765
TATCAAC23500.038.609612
GGTAATC3600.037.859738
GTACATG34250.033.491551
TACATGG35050.032.8612482
AAGGGTA4450.032.740385
ACGCAGA28050.032.5050547
CGCAGAG28300.032.2179078
ACATGGG36250.031.7643623
ACTATAT753.26947E-431.3411371
TACGCTA1201.6512604E-731.3321919
ACCTAAG14400.030.3617231
TATACGC1252.3617213E-730.0789057
GTCTTAG9100.029.9635031
GTAATCA5050.029.7810949
Produced by FastQC (version 0.11.2)