FastQCFastQC Report
Sun 14 Apr 2019
SRR6576956_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576956_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700878
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA43200.6163697533664917No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC24350.34742137718689986No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC22830.3257342932721529No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC17830.25439520144732747No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG12230.17449541860352302No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC12110.1727832803997272No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG11980.17092846401228173No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT10460.1492413800975348No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10380.14809995462833758No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA9550.1362576653854166No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC9140.1304078598557809No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC8860.12641287071359067No Hit
GACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGC8610.12284591612234938No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT8410.11999235244935637No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA8120.11585468512351652No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT8000.11414254691972069No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA7550.10772202865548641No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT7530.10743667228818711No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT7420.10586721226804095No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA7360.10501114316614305No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT7170.10230025767679968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3850.069.755481
GTATCAA18000.049.471321
TCAACGC19800.044.6306344
ATCAACG19800.044.155843
CAACGCA20350.043.1934175
TATCAAC20400.042.8571362
AACGCAG20600.042.4410486
AAGGGTA2950.036.647655
GTACATG33650.036.404281
TACATGG34750.034.763122
CATATAG959.436444E-734.634973
ACGCAGA25400.034.415787
CGCAGAG25650.034.2635738
ACATGGG35750.034.1851653
TAACGCT851.7526341E-533.1797184
GGTAATC3350.032.2672128
ATGGGGG7800.031.3364015
TTAGGTA8300.030.5813084
GCAGAGT28750.030.5690659
CTTAGGT8150.030.5674083
Produced by FastQC (version 0.11.2)