FastQCFastQC Report
Sun 14 Apr 2019
SRR6576958_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576958_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48915
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2080.42522743534703056No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1110.2269242563630788No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT930.19012572830420116No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGA200.002125412570.709231
GGGCGAG200.002151508870.492337
GGCGAGC200.002151508870.492338
GGGGCGA200.002151508870.492336
GTATCAA2450.057.7218171
GTACATC250.00514539856.5673871
CCCTGGC250.005208438756.3938647
ATGGGAA902.401066E-1046.9948845
CATGGGA2650.044.3347974
TATCAAC3250.043.3798942
TACATGG5000.043.2352942
GTACATG5050.042.938941
AACGCAG3200.042.5891156
ATGGGAG1006.7848305E-1042.29545
ACATGGG5000.042.29543
ATGGGGC450.00100498841.773235
ATCAACG3300.041.298543
TCAACGC3300.041.298544
CAACGCA3400.040.0838745
GTGTAGC500.001661725337.711591
Produced by FastQC (version 0.11.2)