FastQCFastQC Report
Sun 14 Apr 2019
SRR6576961_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576961_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1185873
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA41010.34582117983966243No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC25540.21536876208497874No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC22780.19209476900140235No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16390.13821041544920915No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG13720.11569535692270588No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC13720.11569535692270588No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT13360.11265961869441332No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA12530.10566055555696098No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA12240.10321509976194752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6300.059.6862641
GTATCAA24950.057.458491
ATCAACG30300.046.0682953
TCAACGC30350.045.8375434
CAACGCA31650.044.10335
AACGCAG32650.042.7525066
TTAGGTA8350.038.274614
TATCAAC36850.038.2656172
CTTAGGT8800.036.8514673
TAGGTAT8900.035.9093255
GTCTTAG9600.035.2521971
ACGCAGA40950.033.9723937
TCTTAGG11400.033.8091242
AGGTATA9350.033.6784066
CGCAGAG42950.032.281018
GGTATAG10200.030.871877
TTAAGAC3200.030.8430713
TATAGTA11200.030.6332559
GTATAGT11500.030.2428158
GTACATG50400.030.2161711