FastQCFastQC Report
Sun 14 Apr 2019
SRR6576961_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576961_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1185873
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA43170.36403560920941785No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC25670.21646500088963994No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC21220.17893990334546786No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14790.12471824554568658No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT12340.10405836038091767No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC12000.10119127427641915No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG11990.10110694821452214No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6750.057.929771
GTATCAA23050.055.798081
TCAACGC28750.044.1394274
ATCAACG29550.042.944453
ATACGGT450.001013935241.778066
CAACGCA31250.041.059485
AACGCAG31900.040.2228436
CTTAGGT8400.036.928823
GTCTTAG10500.035.8945241
TATCAAC36000.034.9891242
AGGTATA9050.033.7571336
TAGGTAT9350.033.679375
TTAGGTA9100.033.055174
ACGCAGA40600.031.6009967
CTAGACG600.004160897331.3335463
CGCAGAG41550.030.8784718
GTACATG49450.029.7245851
TATAGTA11250.029.659929
GTATAGT11600.029.575298
TCTTAGG11750.029.2001972
Produced by FastQC (version 0.11.2)