FastQCFastQC Report
Sun 14 Apr 2019
SRR6576962_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576962_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1122373
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA41660.3711778526390068No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC25080.2234551258806119No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC22260.1983297887600646No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15870.14139684400818622No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG13670.1217955171765536No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT12680.11297492010231892No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC12540.11172756294030595No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA12000.10691632817254157No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT11790.1050452924295221No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11400.10157051176391449No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC11350.10112502706319557No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6300.061.9199871
GTATCAA24850.058.4420471
TCAACGC29950.047.536044
ATCAACG30550.046.602443
CAACGCA31300.045.4857675
AACGCAG32600.043.6719176
TATCAAC36000.039.9495852
CTTAGGT8000.038.7642333
GGTAATC3450.038.1343358
TAGGTAT8250.037.5895585
AGGTATA8450.036.6998636
ACGCAGA39050.036.5788277
TTAGGTA8650.035.851314
GTAATCA4350.035.645279
CGCAGAG41200.034.7840278
GTCTTAG9800.034.0506671
GCAGAGT42700.033.3420269
ACCTAAG12750.033.176131
TCTTAGG10900.032.77032
CTAAGAC13950.031.6614553