FastQCFastQC Report
Sun 14 Apr 2019
SRR6576962_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576962_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1122373
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA43100.3840078120197118No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC25830.23013739639139572No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC21540.19191480906971212No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14250.12696313970489312No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT12680.11297492010231892No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT12550.11181665988044971No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC12100.10780729757397942No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG11480.10228328728506476No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21950.056.642151
GGTATCA6450.054.7610661
TCAACGC26800.046.1154
CAACGCA27950.044.385735
ATCAACG27750.044.3669473
AACGCAG29250.042.5736856
TATCAAC32550.038.1130942
GTACTAG550.002690348534.2505571
ACGCAGA36350.033.9995087
CGCAGAG37600.033.017728
GTCTTAG9600.032.868051
AGGTATA9200.032.6898886
TAGGTAT9150.031.8413815
CTTAGGT9350.031.6628653
GCAGAGT40450.030.8076329
GTACATG46600.030.2172971
TTAGGTA9800.030.208964
TACATGG47350.030.1699712
ACCTAAG13800.030.0312841
TATAGTA11050.029.3641749
Produced by FastQC (version 0.11.2)