FastQCFastQC Report
Sun 14 Apr 2019
SRR6576966_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576966_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences522317
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA45860.8780108631348396No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC31710.6071025832971165No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC28290.5416251050607198No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG19510.3735279533310231No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC18420.35265940032585574No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC16310.3122624766186052No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA14760.2825870113360277No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA14450.27665191827951224No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT13170.25214572759454507No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT12440.23816954071952473No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT11590.2218958984677887No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA11010.21079153081366298No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC10930.20925989389585253No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT10560.2021760731509792No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT9830.18819988627595885No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC9680.18532806705506427No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT9270.1774784278512857No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA8860.16962878864750716No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG8710.16675696942661258No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8610.1648424232793495No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA8060.1543124194694027No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT7940.152014964092687No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG7570.14493114334781368No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG7430.1422507787416454No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA7390.14148496028274019No Hit
GTTTGGGGCTGACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACG7280.13937895952075083No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG7210.13803877721766666No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA6930.1326780480053301No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT6710.12846604648135135No Hit
GTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCA6670.12770022802244616No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT6620.1267429549488146No Hit
GGGTATATCAAAGAGAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTAT6610.1265515003340883No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA6600.126360045719362No Hit
GGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTC6500.12444549957209892No Hit
CTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCAC6060.11602149652414147No Hit
GATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACAC5990.11468131422105732No Hit
GACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAA5910.11314967730324688No Hit
ATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCA5900.11295822268852058No Hit
ACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAG5880.11257531345906797No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG5800.11104367654125752No Hit
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA5800.11104367654125752No Hit
GAATTATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGT5740.10989494885289966No Hit
ATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCC5640.10798040270563662No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCACA5640.10798040270563662No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG5570.10664022040255247No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCACATAGTT5530.10587440194364725No Hit
GGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAG5470.1047256742552894No Hit
GATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACAGAAAAGGC5470.1047256742552894No Hit
GCATACTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATG5460.1045342196405631No Hit
GGTGAATATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAA5420.10376840118165788No Hit
GGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTC5330.10204530964912112No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2750.082.076921
TAGTCCG250.005242068356.3792655
GTATCAA17800.051.778391
TCAACGC17950.050.7828454
ATCAACG18050.050.7618143
CAACGCA18150.050.218455
TATCAAC18700.049.2863852
AACGCAG18650.048.8721056
CGCAGAG22800.039.9765248
GGTAATC4500.039.6742978
ACGCAGA23100.039.4573487
AAGGGTA4800.038.173465
GCAGAGT24650.036.976269
AGGGTAA5000.036.6465236
GTGCAAG5450.035.375281
CCTTTAT851.7470367E-533.192871
TGCAAGG5550.032.196092
TTAGGTA8000.031.7163724
GTTCTAG600.004148871631.3488241
CTAGACC600.004164461531.3248143
Produced by FastQC (version 0.11.2)