FastQCFastQC Report
Sun 14 Apr 2019
SRR6576969_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576969_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45042
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1460.3241419119932507No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT710.15763065583233427No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT620.13764930509302428No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGATAG200.002149460670.55
GTATCAA1400.067.142861
GGACTGA250.005203500456.3999986
GCTCTAT250.005203500456.3999981
ATGGGGG601.5707628E-647.05
CATGGGG1500.043.866674
GTACATG3750.042.6133351
TACATGG3750.042.6133352
ACATGGG3850.041.5064933
ATGGGGT704.5365923E-640.2857135
AACGCAG2250.039.6888926
TGGGAGT608.6896245E-539.1666686
CAACGCA2450.038.3673485
TGGCAGC250.001693706437.640-41
TCAACGC2500.037.64
TATCAAC2400.037.2083322
ATCAACG2550.035.0196083
ATGGGAG1107.4200216E-834.1818165
CATGGGT702.1519518E-433.571434
CATGGGA1553.8198777E-1133.354844
Produced by FastQC (version 0.11.2)