FastQCFastQC Report
Sun 14 Apr 2019
SRR6576969_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576969_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45042
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2040.4529106167576928No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT890.1975933573109542No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT790.17539185648949868No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAAACT255.5041135E-575.192894
GTATCAA1450.068.138241
ATGGGGC452.1569394E-762.660745
CATGGGG1300.057.8406834
TACATGG3200.057.2758372
TGTTAGG250.005205448756.394672
GTACATG3300.055.6019861
ACATGGG3400.053.9066663
ATGGGAG701.371518E-953.70925
CATGGGA1650.048.4196664
ATGGGGG502.9648863E-546.9955565
TGGGGCC502.9648863E-546.9955566
TCAACGC2100.046.9955564
CAACGCA2150.045.9026345
ATCAACG2150.045.9026343
AACGCAG2150.045.9026346
TATCAAC2250.043.8625142
ATGGGAT652.72724E-643.3805125
GGTATCA459.9857E-441.8202931
CGCAGAG2450.040.2819068
Produced by FastQC (version 0.11.2)