FastQCFastQC Report
Sun 14 Apr 2019
SRR6576978_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576978_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences752680
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC14220.18892490832757614No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA14100.18733060530371473No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC11340.1506616357549025No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8820.11718127225381304No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA7570.1005739490885901No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3650.061.823561
GTATCAA14350.058.3141141
TCAACGC17900.046.2114754
ATCAACG18200.045.4497453
CAACGCA18500.044.7127235
AACGCAG19600.042.2033356
TATCAAC20800.040.0051162
ACGCAGA23800.034.5582127
CGCAGAG24050.034.198988
TACATGG30750.032.717062
GTACATG31450.032.287821
ACATGGG31800.031.3327793
GCAGAGT27000.030.4624239
GTCTTAG4500.030.2964041
CTATATT1005.3217664E-528.1995014
CATGGGG17850.026.8566684
AGAGTAC24100.026.81487810-11
ACCTAAG5800.026.7480131
TAGTACA1258.020201E-626.3195364
TAGGTAT3900.025.3072455
Produced by FastQC (version 0.11.2)