FastQCFastQC Report
Sun 14 Apr 2019
SRR6576980_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576980_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48489
Sequences flagged as poor quality0
Sequence length100
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1270.2619150735218297No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT660.13611334529480915No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA620.12786405164057826No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC580.11961475798634741No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG540.11136546433211655No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC540.11136546433211655No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT510.10517849409144343No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGAG250.00519931456.4179762
GGGAGTA250.005220636356.3597538
CATGGGG2100.051.4394574
GTATCAA1950.050.6315161
ACATGGG3400.048.3478243
GTACATG3350.047.7166981
TACATGG3350.047.7166982
ATGGGGT502.9791763E-546.966465
CAACGCA2000.046.966465
ATCAACG2050.045.8209343
AACGCAG2050.045.8209346
TCAACGC2050.045.8209344
GGGGAGT652.7432798E-643.3536577
TATCAAC2200.042.740892
TGGGGAG901.2776582E-841.7479676
ACGCAGA2350.039.971467
CGCAGAG2400.039.1387188
GGTATCA853.8424878E-738.7182161
GGTAGCA250.001691689737.61198472-73
GCAGAGT2800.033.547479
Produced by FastQC (version 0.11.2)