FastQCFastQC Report
Sun 14 Apr 2019
SRR6576980_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576980_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48489
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2170.44752418074202394No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1160.23922951597269482No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT730.15054960918971313No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC620.12786405164057826No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC620.12786405164057826No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA560.11549011115923198No Hit
GTAAAAGAATATGAAATATAGCCTTGTCTAAATTCTCTTGCTGACTCTCT510.10517849409144343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1400.060.791721
TTCTGTA250.005210434656.3876152
TAAGAGT250.005210434656.3876154
GAATATG352.912613E-453.702497
GTACATG3100.045.757211
TACATGG3100.045.473882
ACATGGG3100.045.473883
GGTATCA652.6192483E-643.645341
ATGGGGG652.7339665E-643.375095
CAGAAGG450.00100544441.7686047
AGAACAG450.00100544441.7686045
TGTAGAT450.00100544441.7686043
CGTTGCC354.598316E-640.2768748-49
CATGGGG1700.038.6973844
TGGGGGC500.001687808737.5917436
CAACGCA2200.036.3102075
ATCAACG2200.036.3102073
TGGGGAG651.3954136E-436.145916
CTCGTTG401.149482E-535.2422646-47
TATCAAC2300.034.7315032