FastQCFastQC Report
Sun 14 Apr 2019
SRR6576987_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576987_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences590221
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA23930.40544135162930495No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC16960.2873499926298793No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC15110.2560058012168323No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC10940.18535429949120755No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7680.130120751379568No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG7290.1235130569735743No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC6950.11775250287604136No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA6860.11622765032081205No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA6830.1157193661357356No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA6390.10826453142128117No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5910.10013198446005818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3550.060.9160161
GTATCAA14700.049.24991
TCAACGC16250.043.383994
ATCAACG16600.042.469273
CAACGCA17000.041.4699945
AACGCAG17800.039.606176
TATCAAC19250.036.8763732
GTACATG29200.036.5464361
TACATGG29650.035.6746562
ACATGGG30150.034.9182363
CGCAGAG21550.032.7141468
CATGGGG17750.032.5685464
ACGCAGA22050.031.9723287
GTATAGA753.2690252E-431.3408471
GGTACCT2650.030.1505078
CTTAGGT5100.029.4897693
GCAGAGT24500.029.1587649
AGGTATA5050.028.8510686
TAGGTAT5300.028.3769475
ACCTAAG8600.027.8787771
Produced by FastQC (version 0.11.2)