FastQCFastQC Report
Sun 14 Apr 2019
SRR6576987_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576987_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences590221
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA24660.4178096001328316No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC15910.269560046152204No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC14570.24685668588545645No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC9680.16400636371799715No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7950.13469530904525592No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC7120.12063277992480782No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6570.1113142365317398No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG6490.10995881203820265No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA6300.10673967886605186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3000.058.141481
GTATCAA13200.057.1415021
TCAACGC16100.046.1261334
ATCAACG16550.044.8719523
CAACGCA16850.044.351995
AACGCAG17150.043.8502166
TATCAAC18800.039.5016372
GTACATG26000.037.169451
TACATGG26050.037.168532
ACGCAGA20900.035.9793057
ACATGGG26850.035.8860323
GTATTCG401.1590188E-535.24847818-19
CGCAGAG21800.034.0627488
GCAGAGT22800.032.7748959
CATGGGG16550.032.0919654
CTTAGGT4900.031.6543713
AGGTATA5050.031.6448766
CCTATAG1052.0624466E-631.3346333
TATTCGA452.5947507E-531.33197620-21
GGTATAG5050.030.711547