FastQCFastQC Report
Sun 14 Apr 2019
SRR6576988_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576988_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539615
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA23260.43104806204423524No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC15490.28705651251355135No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC15290.2833501663222853No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC10250.18995024230238225No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7750.14362091491155732No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG6660.12342132816915764No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA6480.12008561659701825No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC6430.11915903004920175No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6050.11211697228579635No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA5760.10674277030846065No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA5730.10618681837977076No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA5720.10600150107020748No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT5640.10451896259370108No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5490.10173920295025157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3550.075.470291
GTATCAA13200.052.7008131
TCAACGC14600.045.698724
ATCAACG14700.045.3878443
GGTAATC2250.043.8536078
CAACGCA15550.042.9068375
AACGCAG16200.040.895236
AGGGTAA2750.039.2973866
AAGGGTA2800.036.917585
TATCAAC18350.036.6293752
GTAATCA3250.034.697369
GTGCAAG3550.034.4250451
ACCTAAG7600.034.015641
GTACATG25300.033.9985281
TAGACAG1405.11136E-1033.5614365
ACGCAGA19800.033.4597367
TAGGTAT4750.032.642915
CTAAGAC8250.032.463063
TCTTAGG5800.032.4161572
CGCAGAG20450.032.396228
Produced by FastQC (version 0.11.2)