FastQCFastQC Report
Sun 14 Apr 2019
SRR6576991_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576991_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43134
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1040.24110910186859552No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA540.12519126443177075No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC500.11591783743682478No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT500.11591783743682478No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC470.1089627671906153No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.1089627671906153No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA450.1043260536931423No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACCT250.005202519256.3986058
GTATCAA1700.044.2855761
ATGGGGG853.839723E-738.7049265
TATCAAC2250.033.4602132
ATCAACG2300.032.6948473
AACGCAG2300.032.6948476
GTACATG3350.032.305341
TCAACGC2350.031.999214
TACATGG3450.031.3689522
CAACGCA2400.031.3325615
ACATGGG3450.029.9702763
CGCAGAG2750.029.053838
CATGGGG2002.3646862E-1128.1993034
GCAGAGT2850.028.0343979
ACGCAGA2700.027.8511647
GGTATCA856.6649343E-427.6784861
CTTCACT350.00879263226.8564870-71
CATTCAT350.00879263226.8564892-93
TACCTGG350.00879263226.8564810-11
AGAGTAC2700.025.24011810-11
Produced by FastQC (version 0.11.2)