FastQCFastQC Report
Sun 14 Apr 2019
SRR6576994_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576994_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences966472
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA43080.44574493622163913No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC28860.298611858388034No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC27490.28443658998915644No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC17390.17993278646458458No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC15830.16379160493009629No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT13650.14123533842677283No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG13420.13885554884155982No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA11300.11692009701263978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17050.052.6636661
TCAACGC19050.044.655334
ATCAACG19350.043.9633
GGTATCA3850.042.737531
CAACGCA20100.042.556425
AACGCAG21600.039.601116
TATCAAC23050.037.119542
TACATGG37800.035.6937522
GTACATG37550.035.6811
ACATGGG38800.034.4014173
CTTAAGT2000.032.8993843
GGTAATC3600.032.6382758
ACCTAAG14700.031.021051
CTAAGAC15950.030.6451973
CGCAGAG28550.029.960918
ACCAGAT16500.029.90853394
ACGCAGA28650.029.8563337
TAGGTAT8300.029.4452345
TAAGACA18950.029.0179254
GACCGTA650.00615425228.9225357
Produced by FastQC (version 0.11.2)