FastQCFastQC Report
Sun 14 Apr 2019
SRR6576995_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576995_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871927
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA42340.4855911102649648No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC28750.3297294383589452No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC25510.29257036426214583No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC16040.18396035447921674No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC15300.1754734054571082No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT12110.13888777386180265No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG11690.1340708568492546No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA10370.11893197480981779No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA8860.10161401126470448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3750.053.902771
GTATCAA16400.050.4478571
TCAACGC19050.042.423664
ATCAACG19350.041.7659233
CAACGCA19600.041.23325
CTTAGGT7700.039.664073
TTAGGTA8100.039.44564
AACGCAG20700.039.269056
TAGGTAT8100.038.8655175
AGGTATA8000.038.7646
TATCAAC21550.037.7353022
CCTAACG757.4355194E-637.6044272
GTCTTAG9150.037.5038341
GGTATAG9400.033.4904947
GTTATAC851.7521961E-533.182281
GTACATG34650.032.9668121
TATAGTA10750.032.7813959
TACATGG35100.032.2744562
GGTAATC3250.031.806368
ACATGGG35800.031.4994413