FastQCFastQC Report
Sun 14 Apr 2019
SRR6576995_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576995_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871927
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA42280.4849029792631723No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC28640.32846786485565876No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC25270.2898178402549755No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC14650.16801865293768858No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC14610.16755989893649353No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT11540.13235052934477315No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG11060.12684548133043247No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA8820.10115525726350945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15750.054.0943221
TCAACGC17400.047.8029634
ATCAACG17850.046.3345833
CAACGCA18350.045.3281565
AACGCAG19350.043.4713256
TATCAAC20950.039.9270132
GGTATCA3450.038.202571
GTACATG32750.035.7883531
TACATGG33200.035.1030122
GTCTTAG8850.034.571941
TTAGGTA7800.034.3408554
TAGGTAT7800.034.3408555
CGCAGAG24450.033.8464978
ACGCAGA24750.033.4170657
ACATGGG34800.033.3540463
GGTATAG8300.032.8383037
CTTAGGT8300.032.2721253
AGGTATA8450.031.699256
ACCTAAG14350.031.4900261
GTATAGT9600.030.8566848