FastQCFastQC Report
Sun 14 Apr 2019
SRR6576996_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576996_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences910943
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA27690.30397072045122475No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC18710.20539155578340246No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC17370.19068152453007486No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC12870.14128216584352699No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC11690.12832855623238776No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9850.10812970734722151No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9680.10626350935239637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18250.055.6495251
GGTATCA3400.049.7845081
TCAACGC20400.047.9195864
ATCAACG20600.047.6824873
CAACGCA21550.045.362395
AACGCAG22650.043.3668566
TATCAAC23350.042.085262
ACGCAGA28500.034.6301427
GTACATG37050.034.264641
TACATGG37650.033.5937042
CGCAGAG29300.033.3638048
ACATGGG39050.032.0140383
CGTATCC600.00416137731.33203594
GGTACCT4800.030.352918
ATTATCC11950.030.2832643
GCAGAGT33100.029.5335189
GTACCGT804.781273E-429.3737816
ACCTAAG10600.027.9450741
TTAGGTA6500.027.4757844
TATTATC13550.027.4131162
Produced by FastQC (version 0.11.2)