FastQCFastQC Report
Sun 14 Apr 2019
SRR6576996_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576996_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences910943
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA27890.3061662475039602No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC17370.19068152453007486No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC16780.18420471972450525No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC11670.1281090035271142No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC11530.1265721345901994No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9410.10329954783120349No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3200.060.3888781
GTATCAA15950.057.327651
TACTAGT405.675604E-447.0008242
TCAACGC20700.044.276144
ATCAACG20750.043.716433
CAACGCA21550.042.5274165
AACGCAG22650.040.8770566
TATCAAC24150.037.5617332
TTAGGTA6550.035.160924
TAGGTAT6600.034.8926355
CTTAGGT6600.034.182423
ACGCAGA28250.032.2731257
GTACATG37650.032.1729771
TACATGG38050.031.6221052
ACATGGG38850.031.091923
CGCAGAG29500.030.9056228
GTCTTAG8450.030.6781431
AGGTATA7250.030.4678276
CTATATT953.7509817E-529.684734
TATAGTA8250.029.621519
Produced by FastQC (version 0.11.2)