FastQCFastQC Report
Sun 14 Apr 2019
SRR6576997_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576997_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences833939
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA27550.3303598944287292No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC18310.21956042348421165No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC16990.20373192763499487No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC12100.14509454528448723No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC11350.13610108173379587No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA8990.10780164976095373No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8550.10252548447788148No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8350.10012722753103044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16800.052.5964431
GGTATCA4450.048.5853841
TCAACGC19100.044.5296064
CAACGCA19350.044.194485
ATCAACG19200.044.052943
AACGCAG20450.042.0470356
TATCAAC22250.037.8121072
GTACATG34100.034.871771
TTAGGTA6100.034.664614
CTTAGGT6250.034.5844963
TAGGTAT6300.034.307965
TACATGG34950.034.1581542
GGTACCT3200.033.7718968
AGGTATA6450.033.51016
ACATGGG35700.032.7744033
ACGCAGA27000.031.8467377
TAAGGGG1351.3291356E-831.3265384
GGTATAG6900.031.3246617
CGCAGAG27600.031.1544198
GTCTTAG7350.030.6945821
Produced by FastQC (version 0.11.2)