FastQCFastQC Report
Sun 14 Apr 2019
SRR6577002_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6577002_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences607989
Sequences flagged as poor quality0
Sequence length100
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14480.2381622035924992No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA10790.1774703160747974No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8630.14194335752785No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC8400.13816039434923988No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8160.13421295451069015No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC7820.12862074807274473No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA7700.12664702815346987No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7650.125824644853772No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT7620.12533121487395332No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7190.11825871849655174No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA6500.10690982896072133No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT6290.10345581910199034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15000.045.4448661
ATCAACG18800.035.2444463
TCAACGC18950.034.965474
CAACGCA19550.034.1327325
TACACTG2100.033.566145
AACGCAG20450.032.6305586
GTACATG30850.032.611191
GGTATCA7250.032.422021
TACATGG31300.031.8419462
ACATGGG32500.030.6536333
CTTAGGT3000.029.7619783
GTCTTAG4500.029.2518671
GGTACCT5700.028.8551038
TTAGGTA3100.028.8019164
TAGGTAT3300.028.4803625
TATCAAC23800.028.4441932
AGGTATA3000.028.1955596
CGCAGAG23850.027.7817888
GTCTAGG856.802267E-427.6540761
CATGGGG19100.027.5558664
Produced by FastQC (version 0.11.2)