FastQCFastQC Report
Sun 14 Apr 2019
SRR6577002_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6577002_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences607989
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12730.20937878810307425No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA11290.18569414907177598No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9110.14983823720494943No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8940.14704213398597674No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8350.13733801104954202No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC8160.13421295451069015No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC7470.12286406497485974No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT7400.12171272835528274No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7110.11694290521703518No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA6600.10855459556011705No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA6130.1008241925429572No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14550.050.530331
GGTATCA6000.038.4888881
TCAACGC19700.036.7361034
ATCAACG19700.036.497563
CAACGCA19850.036.221765
AACGCAG20250.035.738336
GTACATG29750.032.158781
GGTACCT5050.031.6392468
TACATGG30400.031.5377642
GCTATAG753.2214343E-431.4194981
TGGGTAC5000.031.0157666
TTAAGAC2750.030.7594383
GGGTACC5200.030.7265767
TATCAAC23900.030.6761592
ACATGGG31250.030.0758953
CGCAGAG24300.029.7819428
ACGCAGA24350.029.5277967
CTATAGC804.7797817E-429.3734072
GTTATAA1154.103438E-628.6873681
CTTAAGA3000.028.1984712
Produced by FastQC (version 0.11.2)