FastQCFastQC Report
Sun 14 Apr 2019
SRR6577004_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6577004_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences431104
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA21040.48804928741092635No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC14070.3263713628266033No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC11020.2556227731591449No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC7360.1707244655581948No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC6560.15216745843230406No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT6530.15147157066508316No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG5460.1266515736342043No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT5200.12062054631828978No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT4870.11296578087885986No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA4810.11157400534441805No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA4540.10531101543942993No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT4490.10415120249406176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2050.064.276111
GTATCAA8850.054.2378271
ATCAACG9800.048.9119073
TCAACGC9800.048.9119074
CAACGCA10350.046.7667735
AACGCAG10550.045.88026
TATCAAC10850.044.1784972
TATGTAC652.7833848E-643.3788873
GGTAATC1500.037.616858
ACGCAGA13300.036.0403567
CGCAGAG13450.035.3094988
TAGGTAT3900.034.9441035
GTACTAT550.002698399434.2249371
AAGGGTA1800.033.939965
TAAGCCT702.1844392E-433.5674
TACATGG18850.033.1574252
GTCTTAG4550.033.0966451
GTACATG19750.032.4053841
AGGTATA4250.032.0663536
CTTAGGT4250.032.0663533
Produced by FastQC (version 0.11.2)