FastQCFastQC Report
Sun 14 Apr 2019
SRR6577005_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6577005_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences787486
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA13970.17739997917423292No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12340.1567011984974971No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12320.1564472257284574No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA11220.1424787234312737No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA11090.1408279004325156No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC10920.13866913189567814No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT10610.13473255397556275No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10430.13244679905420542No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10230.12990707136380839No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC9990.12685939813533192No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC9650.12254186106165697No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9420.11962117421770038No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG9400.11936720144866068No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC9190.11670048737374378No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG8410.10679554938119536No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA8230.10450979445983802No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT8190.10400184892175861No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8150.1034939033836792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13550.055.6641731
ATCAACG15800.045.5241283
CAACGCA16500.043.8777245
TCAACGC17650.040.752484
AACGCAG19200.038.441986
GGTATCA4300.038.370251
GTACTAG2000.037.7124751
TATCAAC21200.034.8153762
ACGCAGA21000.034.464417
GTACATG28500.033.7427441
TACATGG28250.033.4491352
CGCAGAG21800.033.4173668
ACATGGG28850.032.5905343
GCAGAGT23850.030.7420779
TTAGGTA3150.029.8487914
TAGGTAT3350.028.0667725
CATGGGG20950.027.6012274
ATGGGGG9850.026.7275495
CTTAGGT3650.025.7599143
AGAGTAC21500.025.3580910-11
Produced by FastQC (version 0.11.2)